The interleaved scatter plot will group together all 6 treatment groups together within each variable. Once your data are entered in this way, you can switch to the graph sheet and select (as you pointed out) an interleaved or separated scatter plot. Make sure you enter the values for the 6-8 subjects in each treatment group in the subcolumns of the appropriate row for the given measured variable Use the rows to represent your variables (so in total, you'll have 10 rows though in the screenshot I thought I counted 11).

Use the main columns to represent your treatment groups (so 6 columns, each with 8 subcolumns).

I'll warn you, this organization is going to be a little different than you'd likely encounter in other packages. If you still need assistance, reach out to me at [ I'll be happy to continue helping you there and you won't have to worry about sharing your data on a public forum.īased on your description, you'll probably want to make a Grouped data table with the option 'Enter 8 replicates in side-by-side subcolumns'.

However, I think that with the amount of data that you have, displaying all of the data on a single graph will be appropriate (and make it easier to analyze the data as well). If you want to make multiple graphs from this data, the easiest solution would be to enter the data for each graph onto separate data tables. However, data from those same 8 individuals for Variable 2 would go in the second row in the same subcolumns A:Y1-A:Y8. In this case, the data for Variable 1 from the 8 individuals in Treatment Group 1 would go in the first row in subcolumns A:Y1-A:Y8. The same idea applies as to how the data would be entered for the individuals. Data from those same 8 individuals for Variable 2 would go in the first row in subcolumns B:Y1-B:Y8Ģ) Use columns to define treatment groups and row to define variables as shown here.

As an example, data for Variable 1 from the 8 individuals in Treatment Group 1 would go in the first row in subcolumns A:Y1-A:Y8. The two options are:ġ) Use columns to define variables and rows to define treatment group as shown here. The instructions I gave you before will generate a single graph.Īs I mentioned in my earlier post, you need to re-arrange your data so that the rows and columns represent the treatment groups and variables.

Graphpad prism 4 parameter fit